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InvivoGen tlr4

Receptor-specific activities of individual PRR ligands and conjugated PRR ligands. HEK-Blue NOD2, TLR2, TLR7, <t>TLR4,</t> or HEK-Lucia RIG-I cells were treated with the compounds (1 or 10 μM as indicated) and the corresponding positive controls (1 μM MDP for NOD2, 100 nM Pam3CSK4 for TLR2, 1 μM imiquimod for TLR7, 100 ng/mL LPS for TLR4, and 100 ng/mL Poly(I/C) for RIG-I) for 18 h. The activities are shown relative to the vehicle-treated control (0.1% DMSO). Data are mean ± SEM of three independent experiments. EC 50 values were determined in HEK-Blue NOD2, TLR2, and TLR7 cells in at least three independent experiments with eight concentrations (1 nM to 10 μM for NOD2 cells, and 0.1 nM to 1 μM for TLR2 and TLR7 cells).

Tlr4, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek+tlr4+cells/HEK-Blue+hTLR4+cells/pmc13224164-370-9-20
Average 97 stars, based on 432 article reviews

tlr4 - by Bioz Stars, 2026-10

97/100 stars

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1) Product Images from "Probing Immune Signatures of Conjugated Pattern Recognition Receptor Ligands Identifies Chimeras with Potent Adjuvant and Antitumor Activities"

Article Title: Probing Immune Signatures of Conjugated Pattern Recognition Receptor Ligands Identifies Chimeras with Potent Adjuvant and Antitumor Activities

Journal: Journal of Medicinal Chemistry

doi: 10.1021/acs.jmedchem.6c00372

Receptor-specific activities of individual PRR ligands and conjugated PRR ligands. HEK-Blue NOD2, TLR2, TLR7, TLR4, or HEK-Lucia RIG-I cells were treated with the compounds (1 or 10 μM as indicated) and the corresponding positive controls (1 μM MDP for NOD2, 100 nM Pam3CSK4 for TLR2, 1 μM imiquimod for TLR7, 100 ng/mL LPS for TLR4, and 100 ng/mL Poly(I/C) for RIG-I) for 18 h. The activities are shown relative to the vehicle-treated control (0.1% DMSO). Data are mean ± SEM of three independent experiments. EC 50 values were determined in HEK-Blue NOD2, TLR2, and TLR7 cells in at least three independent experiments with eight concentrations (1 nM to 10 μM for NOD2 cells, and 0.1 nM to 1 μM for TLR2 and TLR7 cells).

Figure Legend Snippet: Receptor-specific activities of individual PRR ligands and conjugated PRR ligands. HEK-Blue NOD2, TLR2, TLR7, TLR4, or HEK-Lucia RIG-I cells were treated with the compounds (1 or 10 μM as indicated) and the corresponding positive controls (1 μM MDP for NOD2, 100 nM Pam3CSK4 for TLR2, 1 μM imiquimod for TLR7, 100 ng/mL LPS for TLR4, and 100 ng/mL Poly(I/C) for RIG-I) for 18 h. The activities are shown relative to the vehicle-treated control (0.1% DMSO). Data are mean ± SEM of three independent experiments. EC 50 values were determined in HEK-Blue NOD2, TLR2, and TLR7 cells in at least three independent experiments with eight concentrations (1 nM to 10 μM for NOD2 cells, and 0.1 nM to 1 μM for TLR2 and TLR7 cells).

Techniques Used: Control

Selection:

Article Title: Candida albicans-derived mannoproteins activate NF-κB in reporter cells expressing TLR4, MD2 and CD14.
Article Snippet: The HEK-Null and HEK-TLR4 cell lines were cultured at 37 ̊C/5% CO2 in DMEM supplemented PLOS ONE | https://doi.org/10.1371/journal.pone.0189939 December 27, 2017 16 / 23 with 4.5 g glucose/L (Thermo Fisher), 50 U/mL penicillin, 50 μg/mL streptomycin (Thermo Fisher), 100 μg/mL normocin (Thermo Fisher), 2 mM L-glutamine (Thermo Fisher), and 10% heat-inactivated FBS (Atlanta Biologicals, Flowery Branch, GA). .. HEK-Null and HEK-TLR4 cells were maintained under selection using 100 μg/mL zeocin (InvivoGen) or HEK-BlueTM (InvivoGen), respectively. ..

Expressing:

Article Title: UEG Week 2020 Poster Presentations.
Article Snippet: .. The following 39 inflammatory biomarkers were measured (Meso Scale Discovery, cat# K15209D-2): CRP, Eotaxin, Eotaxin-3, FGF(basic), Flt-1, GMCSF, ICAM-1, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12/IL23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IP-10, MCP-1, MCP-4, MDC, MIP1α, MIP-1β, PlGF, SAA, TARC, TNF-α, TNF-β, Tie-2, VCAM-1, VEGF, VEGF-C, VEGF-D. Pro-inflammatory capacity of endogenous molecules (e.g., endotoxins) was determined using HEK-TLR4 cells expressing human TLR4 (Invivogen, cat# hkb-htlr4), which release secreted embryonic alkaline phosphatase (SEAP) in response to TLR4 activation. ..

Article Title: Axenic Caenorhabditis elegans antigen protects against development of type-1 diabetes in NOD mice.
Article Snippet: .. The HEK TLR4 cells were incubated in 96-well plates at 0.25 106 cells/ml in the presence of purified LPS (10 ng/ml; Invivogen, Product code: tlrl-smlps), CeAg (10 μg/ml), or aCeAg (10 μg/ml) for 20 h at 37 C. To detect NF-κB induced expression of SEAP, using the QUANTI-blue colorimetric assay (InvivoGen, Product code: rep-qbs), 20 μl of cell culture supernatant was incubated with 180 μl of substrate at 37 C for 30 min. ..

Activation Assay:

Concentration Assay:

Article Title: Cell-Derived Vesicles for Antibiotic Delivery-Understanding the Challenges of a Biogenic Carrier System.
Article Snippet: .. Ultrapure LPS from E. coli K12 (Invivogen, #tlrl-peklps) and Pam3CSK4 (#tlrl-pms) were used at concentration of 10 ng mL−1 as positive controls for HEK-TLR2 and HEK-TLR4 cells respectively. .. After 24 and 16 h of incubation for HEK-Dual hTLR2 and HEK-Blue hTLR4, respectively, 20 μL of cell culture supernatant from each well was mixed with 180 μL of resuspended QUANTI-Blue Solution (Invivogen, #REP-QB2) and incubated at 37 °C for 1–3 h. SEAP activity was measured with microplate reader (PromegaTM GloMax Plate Reader Madison, WI, USA) at 600 nm.

Incubation:

Article Title: Axenic Caenorhabditis elegans antigen protects against development of type-1 diabetes in NOD mice
Article Snippet: .. The HEK TLR4 cells were incubated in 96-well plates at 0.25 × 10 6 cells/ml in the presence of purified LPS (10 ng/ml; Invivogen, Product code: tlrl-smlps), CeAg (10 μg/ml), or aCeAg (10 μg/ml) for 20 h at 37 °C. .. To detect NF-κB induced expression of SEAP, using the QUANTI-blue colorimetric assay (InvivoGen, Product code: rep-qbs), 20 μl of cell culture supernatant was incubated with 180 μl of substrate at 37 °C for 30 min.

Purification:

Colorimetric Assay:

Cell Culture:

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Journal: Journal of Medicinal Chemistry

Article Title: Probing Immune Signatures of Conjugated Pattern Recognition Receptor Ligands Identifies Chimeras with Potent Adjuvant and Antitumor Activities

doi: 10.1021/acs.jmedchem.6c00372

Figure Lengend Snippet: Receptor-specific activities of individual PRR ligands and conjugated PRR ligands. HEK-Blue NOD2, TLR2, TLR7, TLR4, or HEK-Lucia RIG-I cells were treated with the compounds (1 or 10 μM as indicated) and the corresponding positive controls (1 μM MDP for NOD2, 100 nM Pam3CSK4 for TLR2, 1 μM imiquimod for TLR7, 100 ng/mL LPS for TLR4, and 100 ng/mL Poly(I/C) for RIG-I) for 18 h. The activities are shown relative to the vehicle-treated control (0.1% DMSO). Data are mean ± SEM of three independent experiments. EC 50 values were determined in HEK-Blue NOD2, TLR2, and TLR7 cells in at least three independent experiments with eight concentrations (1 nM to 10 μM for NOD2 cells, and 0.1 nM to 1 μM for TLR2 and TLR7 cells).

Article Snippet: HEK-Blue NOD2 (Cat. code: hkb-hnod2), TLR2 (Cat. code: hkb-htlr2), TLR4 (Cat. code: hkb-htlr4), and TLR7 (Cat. code: hkb-htlr7) cell lines (Invivogen, San Diego, CA) are derived from HEK293 cells by cotransfection of hNOD2, hTLR2, hTLR4, or hTLR7 genes, respectively, and a nuclear factor-κB (NF-κB)-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene.

Techniques: Control

Microbiome assessment and TLR2 and 4 ligands at the Run-in phase (A) PCoA based on phylo-RPCA distances. Locations are differentiated by markers and weeks by color. (B) Shannon entropy and Faith phylogenetic diversity boxplots by week, location, group, and sex. The line inside the box represents the median, while the whiskers represent the lowest and highest values (excluding outliers) and interquartile range (IQR). Independent samples were tested with the Kruskal-Wallis test, and dependent samples were tested with the Wilcoxon test. Significant p -values are indicated with asterisks (∗ - p < 0.05; ∗∗ - p < 0.01; ∗∗∗ - p < 0.001). (C) ANCOM-BC differential abundance test between locations. Colors indicate where a given taxon was found to be more abundant. Log fold changes plotted only for taxons with adjusted p -values less than 0.05. (D) Toll-like receptor 4 (TLR4) (Wilcoxon test, n = 34) and (E) TLR2 ligands (Wilcoxon test, n = 34) in the serum of participants at timepoint -3W and 0W. Single data points are presented in graph D and E.

Journal: iScience

Article Title: Fiber enrichment is not superior to dietary monitoring in MASLD: A dual-center, double-blind, placebo-controlled trial

doi: 10.1016/j.isci.2025.114019

Figure Lengend Snippet: Microbiome assessment and TLR2 and 4 ligands at the Run-in phase (A) PCoA based on phylo-RPCA distances. Locations are differentiated by markers and weeks by color. (B) Shannon entropy and Faith phylogenetic diversity boxplots by week, location, group, and sex. The line inside the box represents the median, while the whiskers represent the lowest and highest values (excluding outliers) and interquartile range (IQR). Independent samples were tested with the Kruskal-Wallis test, and dependent samples were tested with the Wilcoxon test. Significant p -values are indicated with asterisks (∗ - p < 0.05; ∗∗ - p < 0.01; ∗∗∗ - p < 0.001). (C) ANCOM-BC differential abundance test between locations. Colors indicate where a given taxon was found to be more abundant. Log fold changes plotted only for taxons with adjusted p -values less than 0.05. (D) Toll-like receptor 4 (TLR4) (Wilcoxon test, n = 34) and (E) TLR2 ligands (Wilcoxon test, n = 34) in the serum of participants at timepoint -3W and 0W. Single data points are presented in graph D and E.

Article Snippet: Human TLR4 Reporter HEK293 Cell Assay , Invivogen , Cat# hkb-htlr4, RRID:CVCL_IM82.

Techniques:

TLR2 and 4 ligands during dietary fiber and placebo intervention Linear mixed effects analyses were performed for intervention (weeks 0–12) and follow-up (weeks 12–20) stages with the placebo group as reference. (A) Toll-like receptor 4 (TLR4, oat bran n = 12 expect for 20W n = 11, spelt bran n = 11 expect for 20W n = 10, placebo n = 11) and (B) TLR2 ligands (oat bran n = 12 expect for 20W n = 11, spelt bran n = 11 expect for 20W n = 10, placebo n = 11) in serum of patients at timepoint 0W to 20W. Bands represent 95% confidence intervals. p -values and coefficients are plotted on the right side of the subplots.

Journal: iScience

Article Title: Fiber enrichment is not superior to dietary monitoring in MASLD: A dual-center, double-blind, placebo-controlled trial

doi: 10.1016/j.isci.2025.114019

Figure Lengend Snippet: TLR2 and 4 ligands during dietary fiber and placebo intervention Linear mixed effects analyses were performed for intervention (weeks 0–12) and follow-up (weeks 12–20) stages with the placebo group as reference. (A) Toll-like receptor 4 (TLR4, oat bran n = 12 expect for 20W n = 11, spelt bran n = 11 expect for 20W n = 10, placebo n = 11) and (B) TLR2 ligands (oat bran n = 12 expect for 20W n = 11, spelt bran n = 11 expect for 20W n = 10, placebo n = 11) in serum of patients at timepoint 0W to 20W. Bands represent 95% confidence intervals. p -values and coefficients are plotted on the right side of the subplots.

Article Snippet: Human TLR4 Reporter HEK293 Cell Assay , Invivogen , Cat# hkb-htlr4, RRID:CVCL_IM82.

Techniques:

Microbiome composition of responder and non-responder subgroups and correlations with TLR2/TLR4 ligands (A) MaAsLin2 correlations between microbiota abundances and TLR2/TLR4 ligands in responder (R) and non-responder (N) subgroups within each group. (B–D) PcoA plots based on phylo-RPCA distances within each group. Responder (R) and non-responder subgroups are differentiated by markers and phases by color and marker size. In the right lower corner of each PCoA plot, response rate is plotted. Respond rate was calculated as the number of responders divided by the total number of participants within each group.

Journal: iScience

Article Title: Fiber enrichment is not superior to dietary monitoring in MASLD: A dual-center, double-blind, placebo-controlled trial

doi: 10.1016/j.isci.2025.114019

Figure Lengend Snippet: Microbiome composition of responder and non-responder subgroups and correlations with TLR2/TLR4 ligands (A) MaAsLin2 correlations between microbiota abundances and TLR2/TLR4 ligands in responder (R) and non-responder (N) subgroups within each group. (B–D) PcoA plots based on phylo-RPCA distances within each group. Responder (R) and non-responder subgroups are differentiated by markers and phases by color and marker size. In the right lower corner of each PCoA plot, response rate is plotted. Respond rate was calculated as the number of responders divided by the total number of participants within each group.

Article Snippet: Human TLR4 Reporter HEK293 Cell Assay , Invivogen , Cat# hkb-htlr4, RRID:CVCL_IM82.

Techniques: Marker

Chemical structures of the first reported conjugated NOD2/TLR4 agonists 1 and 2 .

Journal: ACS Omega

Article Title: Modulating Receptor Activity, Immune Response, and Kinetic Solubility: The Impact of Linker Chemistry in Conjugated NOD2/TLR4 Agonists

doi: 10.1021/acsomega.5c05358

Figure Lengend Snippet: Chemical structures of the first reported conjugated NOD2/TLR4 agonists 1 and 2 .

Article Snippet: HEK-Blue hNOD2 cells (25,000 cells/well) and TLR4 cells (40,000 cells/well) were seeded in 96-well plates in 100 μL of HEK-Blue detection medium (Invivogen, San Diego, CA, USA) and treated with compounds (at 0.5 μM, 1 μM, 5 μM and 10 μM in the case of HEK-Blue hNOD2 cells (all conjugates) or with the corresponding vehicle (0.1% DMSO) and in the case of HEK-Blue hTLR4 cells at two different concentration ranges, 0.5 μM, 1 μM, 5 μM and 10 μM (conjugates 17–21 ) and 1 μM, 10 μM and 100 μM (conjugates 27–31 ) or the corresponding vehicle (0.1% DMSO).

Techniques:

Design of novel conjugated NOD2/TLR4 agonists.

Journal: ACS Omega

Article Title: Modulating Receptor Activity, Immune Response, and Kinetic Solubility: The Impact of Linker Chemistry in Conjugated NOD2/TLR4 Agonists

doi: 10.1021/acsomega.5c05358

Figure Lengend Snippet: Design of novel conjugated NOD2/TLR4 agonists.

Techniques:

Synthesis of Conjugated NOD2/TLR4 Agonists 17–21 Based on the TLR4 Agonist 4

Journal: ACS Omega

Article Title: Modulating Receptor Activity, Immune Response, and Kinetic Solubility: The Impact of Linker Chemistry in Conjugated NOD2/TLR4 Agonists

doi: 10.1021/acsomega.5c05358

Figure Lengend Snippet: Synthesis of Conjugated NOD2/TLR4 Agonists 17–21 Based on the TLR4 Agonist 4

Techniques:

Synthesis of Conjugated NOD2/TLR4 Agonists 27–31 Based on TLR4 Agonist 6

Journal: ACS Omega

Article Title: Modulating Receptor Activity, Immune Response, and Kinetic Solubility: The Impact of Linker Chemistry in Conjugated NOD2/TLR4 Agonists

doi: 10.1021/acsomega.5c05358

Figure Lengend Snippet: Synthesis of Conjugated NOD2/TLR4 Agonists 27–31 Based on TLR4 Agonist 6

Techniques:

Concentration-dependent NF-κB transcriptional activities of conjugated NOD2/TLR4 agonists. SEAP activity was measured in (A) HEK-Blue hNOD2 cells after incubation for 18 h with MDP (positive control; 4 μM), and compounds (0.5–10 μM); and (B) HEK-Blue hTLR4 (B) cells after incubation for 18 h with LPS (positive control; 10 ng/mL) and compounds (0.5–10 μM or 1–100 μM concentration range as indicated). Data are shown as relative activities to the vehicle-treated control (0.1% DMSO) and are means ± SEM of three independent experiments.

Journal: ACS Omega

Article Title: Modulating Receptor Activity, Immune Response, and Kinetic Solubility: The Impact of Linker Chemistry in Conjugated NOD2/TLR4 Agonists

doi: 10.1021/acsomega.5c05358

Figure Lengend Snippet: Concentration-dependent NF-κB transcriptional activities of conjugated NOD2/TLR4 agonists. SEAP activity was measured in (A) HEK-Blue hNOD2 cells after incubation for 18 h with MDP (positive control; 4 μM), and compounds (0.5–10 μM); and (B) HEK-Blue hTLR4 (B) cells after incubation for 18 h with LPS (positive control; 10 ng/mL) and compounds (0.5–10 μM or 1–100 μM concentration range as indicated). Data are shown as relative activities to the vehicle-treated control (0.1% DMSO) and are means ± SEM of three independent experiments.

Techniques: Concentration Assay, Activity Assay, Incubation, Positive Control, Control

A Inverted binding mode of lipid A moiety of E. coli LPS in TLR4/MD-2 complex. B . Inverted binding mode of A. baumannii acyl 6 lipid A ( 2 ) in TLR4/MD-2 complex. C . Retained binding mode of IV A ( 6 ) in MD-2 receptor.

Journal: Communications Chemistry

Article Title: Synthesis of Acinetobacter baumannii Lipid A(s) and derivatives and their structure-immunostimulatory activity relationships

doi: 10.1038/s42004-025-01628-6

Figure Lengend Snippet: A Inverted binding mode of lipid A moiety of E. coli LPS in TLR4/MD-2 complex. B . Inverted binding mode of A. baumannii acyl 6 lipid A ( 2 ) in TLR4/MD-2 complex. C . Retained binding mode of IV A ( 6 ) in MD-2 receptor.

Article Snippet: To validate which of the TLR(s) is responsible for the NF-κB activation, we examined the stimulatory property with human embryonic kidney (HEK) cells that had been specifically transfected with either human TLR2 or TLR4 gene alongside with the human MD-2 and CD14 genes, i.e., HEK-BlueTM h TLR4 or h TLR2 cell lines (InvivoGen), respectively.

Techniques: Binding Assay

A Binding modes of lipid A moiety of E. coli LPS. B Binding mode of A. baumannii acyl 6 lipid A ( 2 ). C Binding mode of A. baumannii acyl 7 lipid A ( 1 ). D Binding mode of E. coli acyl 6 lipid A ( 5 ). E Binding mode of A. baumannii symmetric acyl 6 lipid A derivative ( 4 ). F Binding mode of S . Typhi acyl 7 lipid A ( 7 ). Cationic residues of TLR4, MD-2, and TLR4* are shown in blue, wheat, and green, respectively. The distances between the PO 4 groups and the residues are depicted by black dotted lines.

Journal: Communications Chemistry

Article Title: Synthesis of Acinetobacter baumannii Lipid A(s) and derivatives and their structure-immunostimulatory activity relationships

doi: 10.1038/s42004-025-01628-6

Figure Lengend Snippet: A Binding modes of lipid A moiety of E. coli LPS. B Binding mode of A. baumannii acyl 6 lipid A ( 2 ). C Binding mode of A. baumannii acyl 7 lipid A ( 1 ). D Binding mode of E. coli acyl 6 lipid A ( 5 ). E Binding mode of A. baumannii symmetric acyl 6 lipid A derivative ( 4 ). F Binding mode of S . Typhi acyl 7 lipid A ( 7 ). Cationic residues of TLR4, MD-2, and TLR4* are shown in blue, wheat, and green, respectively. The distances between the PO 4 groups and the residues are depicted by black dotted lines.

Techniques: Binding Assay

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tlr4 (InvivoGen)

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1) Product Images from "Probing Immune Signatures of Conjugated Pattern Recognition Receptor Ligands Identifies Chimeras with Potent Adjuvant and Antitumor Activities"

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